Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HUVEC_H3K4me3 minus
cell type
human umbilical vein endothelial cells
stimulation
none
chip antibody
H3K4me3 (kindly provided by Dr. Kimura, Osaka University)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were extracted from fixed cells with paraformaldehyde and sonicated protein-DNA complexes were isolated with antibody. Libraries were prepared using ChIP-Seq DNA Sample Prep Kit (Illumina) according to the instruction manuals.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg38

Number of total reads
51999257
Reads aligned (%)
79.6
Duplicates removed (%)
58.0
Number of peaks
18009 (qval < 1E-05)

hg19

Number of total reads
51999257
Reads aligned (%)
79.1
Duplicates removed (%)
59.1
Number of peaks
17912 (qval < 1E-05)

Base call quality data from DBCLS SRA