Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
PC3-HIF1A-ChIP-Seq-siOC2
tumor type
prostate cancer
cell line
PC3
antibody
HIF1A
protocol
siOC2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-enriched DNA was used for library creation with the Rubicon ThruPLEX-FD kit and sequenced (75 bp single-end reads).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
54624212
Reads aligned (%)
13.2
Duplicates removed (%)
86.3
Number of peaks
3921 (qval < 1E-05)

hg38

Number of total reads
54624212
Reads aligned (%)
14.2
Duplicates removed (%)
85.3
Number of peaks
3924 (qval < 1E-05)

Base call quality data from DBCLS SRA