Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
PC3-HIF1A-ChIP-Seq-siCtrl
tumor type
prostate cancer
cell line
PC3
antibody
HIF1A
protocol
siCtrl

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-enriched DNA was used for library creation with the Rubicon ThruPLEX-FD kit and sequenced (75 bp single-end reads).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
66504603
Reads aligned (%)
42.0
Duplicates removed (%)
55.4
Number of peaks
47681 (qval < 1E-05)

hg38

Number of total reads
66504603
Reads aligned (%)
43.2
Duplicates removed (%)
54.4
Number of peaks
48024 (qval < 1E-05)

Base call quality data from DBCLS SRA