Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Erythroid Cells
MeSH Description
The series of cells in the red blood cell lineage at various stages of differentiation.

Attributes by original data submitter

Sample

source_name
Ter119+ Erythroid cells
strain
C57BL6
tissue
Phenylhydrazine treated spleen
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
A single cell suspension was made by gently dissociating the spleen of a phenyhydrazine treated mouse and passing it through a 70um filter. Ter119 selection was performed by staining the cells with phycoerythrin conjugated anti-ter119 antibodies (BD biosciences). The cells were subsequently washed and then incubated with MACS anti-phycoerythrin beads. Cells were then separated using an auto-MACS (Miltenyi Biotec). NEBnext ultra library prep kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
42718485
Reads aligned (%)
96.5
Duplicates removed (%)
24.5
Number of peaks
5760 (qval < 1E-05)

mm9

Number of total reads
42718485
Reads aligned (%)
96.3
Duplicates removed (%)
25.3
Number of peaks
5726 (qval < 1E-05)

Base call quality data from DBCLS SRA