Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CREBBP

Cell type

Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
CBP ChIP-seq from Proliferating Fibroblasts
cell line
IMR90
cell type
Lung Fibroblasts
population doubling
PD25
senescence induction
None
target
CBP
chip antibody
Cell Signaling Technology 7389, RRID: AB_10949313

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described in Shah et al., Genes & Development 2013 from ~12e6 cells. Briefly, cross-linked cells were lysed and the chromatin was sheared using a Covaris Ultrasonicator to achieve an average size of <500 bp. 2ug of sonicated chromatin (estimated by DNA concentration) was used per immunoprecipitation reaction, and 10% of the amount was saved as input. Immunoprecipitation was performed using protein A Dynabeads (Thermo Fisher). 1ng of DNA from immunoprecipitation and input was used to prepare libraries using the NEBNext Ultra II kit (New England Biolabs). The libraries were checked for quality and quantity by Bioanalyzer (Agilent) and qPCR (Kapa Biosystems) respectively. Multiplexed libraries were sequenced on a single lane of the NextSeq 500 platform (Illumina).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
69919976
Reads aligned (%)
50.0
Duplicates removed (%)
47.4
Number of peaks
10442 (qval < 1E-05)

hg38

Number of total reads
69919976
Reads aligned (%)
51.7
Duplicates removed (%)
46.1
Number of peaks
10734 (qval < 1E-05)

Base call quality data from DBCLS SRA