GSM2830401: Input from Proliferating Fibroblasts, Replicate #3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
Input from Proliferating Fibroblasts
cell line
IMR90
cell type
Lung Fibroblasts
population doubling
PD29
senescence induction
None
target
none
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described in Shah et al., Genes & Development 2013 from ~12e6 cells. Briefly, cross-linked cells were lysed and the chromatin was sheared using a Covaris Ultrasonicator to achieve an average size of <500 bp. 2ug of sonicated chromatin (estimated by DNA concentration) was used per immunoprecipitation reaction, and 10% of the amount was saved as input. Immunoprecipitation was performed using protein A Dynabeads (Thermo Fisher). 1ng of DNA from immunoprecipitation and input was used to prepare libraries using the NEBNext Ultra II kit (New England Biolabs). The libraries were checked for quality and quantity by Bioanalyzer (Agilent) and qPCR (Kapa Biosystems) respectively. Multiplexed libraries were sequenced on a single lane of the NextSeq 500 platform (Illumina).