Antigen

Antigen Class

TFs and others

Antigen

PADI2

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

T47D PADI2 T0

cell line

T47D

r5020 treatment

Untreated

chip antibody

PADI2 antibody (z-22)

chip antibody vendor

Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

PADI2 was immunoprecipitated with 6μg of PADI2 antibody (z-22):sc-133877 lot number E1214 and H0715 by 50 μg of chromatin and 42μl of Protein A-Agarose Beads (Diagenode). T47D-MTVL cells, without treatment or transfected with -amanitin resistant HA-tagged wild type (WTr) versus R1810Ar mutant RNAP2 were cross-linked for 10 minutes with 1% formaldehyde. Lysate were sonicated to a DNA fragment size range of 200-300bp using a Biorupter sonicator (Diagenode). PADI2 was immunoprecipitated with 6μg of PADI2 antibody (z-22):sc-133877 lot number E1214 and H0715 by 50 μg and for RNAP2, 150 μg chromatin was incubated with 20μg of anti-HA (ab9110) antibody followed by 42μl of Protein A-Agarose Beads (Diagenode). Libraries were prepared using standadr Illumina instruction of NEBNext® ChIP-Seq Library Prep Reagent.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

58856191

Reads aligned (%)

88.6

Duplicates removed (%)

46.3

Number of peaks

1611 (qval < 1E-05)

hg19

Number of total reads

58856191

Reads aligned (%)

88.0

Duplicates removed (%)

47.3

Number of peaks

2541 (qval < 1E-05)