Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Treg
NA
NA

Attributes by original data submitter

Sample

source_name
Regulatory T cells
strain
C57BL/6
tissue
Spleen
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were sonicated and histone-DNA complexes were isolated with antibody. Libraries were prepared by using DNA SMART ChIP-Seq kit (Clontech) according to the protocol. 150-400bp products were selected and subjected to the analysis.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
7676855
Reads aligned (%)
93.6
Duplicates removed (%)
11.8
Number of peaks
142 (qval < 1E-05)

mm9

Number of total reads
7676855
Reads aligned (%)
93.3
Duplicates removed (%)
11.7
Number of peaks
142 (qval < 1E-05)

Base call quality data from DBCLS SRA