Antigen

Antigen Class

TFs and others

Antigen

Trim28

Cell type

Cell type Class

Blood

Cell type

Treg

NA

NA

Sample

source_name

Regulatory T cells

strain

C57BL/6

tissue

Spleen

antibody

KAP1(active motif, 1Tb-A9)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclear lysates were sonicated and histone-DNA complexes were isolated with antibody. Libraries were prepared by using DNA SMART ChIP-Seq kit (Clontech) according to the protocol. 150-400bp products were selected and subjected to the analysis.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

23128462

Reads aligned (%)

87.9

Duplicates removed (%)

73.3

Number of peaks

4023 (qval < 1E-05)

mm9

Number of total reads

23128462

Reads aligned (%)

87.7

Duplicates removed (%)

73.4

Number of peaks

3977 (qval < 1E-05)