Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

undifferentiated hESC

chip antibody

none

cell line

H9

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using NEBNext® ChIP-Seq Library Prep kit for Illumina (New England Biolabs, #E6200) according to manufacturer's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

18646496

Reads aligned (%)

89.9

Duplicates removed (%)

1.8

Number of peaks

981 (qval < 1E-05)

hg19

Number of total reads

18646496

Reads aligned (%)

89.2

Duplicates removed (%)

3.2

Number of peaks

1044 (qval < 1E-05)