Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA

Attributes by original data submitter

Sample

source_name
undifferentiated hESC
chip antibody
none
cell line
H9

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using NEBNext® ChIP-Seq Library Prep kit for Illumina (New England Biolabs, #E6200) according to manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
44091221
Reads aligned (%)
96.6
Duplicates removed (%)
2.3
Number of peaks
1421 (qval < 1E-05)

hg19

Number of total reads
44091221
Reads aligned (%)
95.9
Duplicates removed (%)
3.8
Number of peaks
1439 (qval < 1E-05)

Base call quality data from DBCLS SRA