Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

Differentiated hESCs

NA

NA

Sample

source_name

trophoblast progenitors at day 2.5

chip antibody

anti-H3K4me3 (C15410003-50; LotNr: A.5051–001P; Diagenode)

cell line

H9

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using NEBNext® ChIP-Seq Library Prep kit for Illumina (New England Biolabs, #E6200) according to manufacturer's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

49359537

Reads aligned (%)

98.6

Duplicates removed (%)

63.6

Number of peaks

27607 (qval < 1E-05)

hg19

Number of total reads

49359537

Reads aligned (%)

98.2

Duplicates removed (%)

64.4

Number of peaks

27744 (qval < 1E-05)