SRX3302801
GSM2825174: H3K4me3 ChIP from SSEA5-positive hESC, biological replicate 2; Homo sapiens; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA

Attributes by original data submitter

Sample

source_name
undifferentiated hESC
chip antibody
anti-H3K4me3 (C15410003-50; LotNr: A.5051–001P; Diagenode)
cell line
H9

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using NEBNext® ChIP-Seq Library Prep kit for Illumina (New England Biolabs, #E6200) according to manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2500

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
46313911
Reads aligned (%)
90.0
Duplicates removed (%)
78.3
Number of peaks
28830 (qval < 1E-05)

hg19

Number of total reads
46313911
Reads aligned (%)
89.7
Duplicates removed (%)
78.9
Number of peaks
28663 (qval < 1E-05)

Base call quality data from DBCLS SRA