Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Egr2

Cell type

Cell type Class
Blood
Cell type
T cells
NA
NA

Attributes by original data submitter

Sample

source_name
T cells
strain
C57BL/6
cell type
T cells
tissue
Spleen
chip antibody
Anti-EGR2 (Covance, PRB-236P D13BF00486)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, chromatin was prepared from cells cross-linked with 1% formaldehyde at 37oC for 15 min. Chromatin was fragmented by sonication and the ChIP DNA was blunt-ended, ligated to the Solexa adaptor and amplified using adaptor primers for 17 cycles. Fragments around 200bp were isolated from agarose gel and purified DNA was used directly for cluster generation and sequencing following the manufacturer protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
28586972
Reads aligned (%)
65.8
Duplicates removed (%)
80.9
Number of peaks
279 (qval < 1E-05)

mm9

Number of total reads
28586972
Reads aligned (%)
65.5
Duplicates removed (%)
81.1
Number of peaks
310 (qval < 1E-05)

Base call quality data from DBCLS SRA