GSM1198165: Total T EGR2 0h; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Egr2
Cell type
Cell type Class
Blood
Cell type
T cells
NA
NA
Attributes by original data submitter
Sample
source_name
T cells
strain
C57BL/6
cell type
T cells
tissue
Spleen
chip antibody
Anti-EGR2 (Covance, PRB-236P D13BF00486)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, chromatin was prepared from cells cross-linked with 1% formaldehyde at 37oC for 15 min. Chromatin was fragmented by sonication and the ChIP DNA was blunt-ended, ligated to the Solexa adaptor and amplified using adaptor primers for 17 cycles. Fragments around 200bp were isolated from agarose gel and purified DNA was used directly for cluster generation and sequencing following the manufacturer protocols.