Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived trophoblast cells
NA
NA

Attributes by original data submitter

Sample

source_name
trophoblast progenitors at day 3_GATA3 ChIP
cell line
H9
source cell type
Human embryonic stem cells (hESC)
treated with
BMP4 for 3 day
cell type
trophoblast progenitors at day 3
chip antibody
anti-GATA3 (#CM405A, Biocare Medical)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP'ed DNA fragments were prepared using High Sensitivity ChIP kit (Active Motif, #53040) according to manufacturer's instrcutions using aforementioned antibodies Libraries were prepared using NEBNext® ChIP-Seq Library Prep kit for Illumina (New England Biolabs, #E6200) according to manufacturer's instructions

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
12917679
Reads aligned (%)
93.3
Duplicates removed (%)
4.2
Number of peaks
12783 (qval < 1E-05)

hg38

Number of total reads
12917679
Reads aligned (%)
95.0
Duplicates removed (%)
3.0
Number of peaks
12824 (qval < 1E-05)

Base call quality data from DBCLS SRA