Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMC3

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
SMC3 (ESCO2 WT; control siRNA)
cell line
HeLa
antibody
Anti-SMC3 (A845; JM Peters lab; Sumara et al., 2002)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples were formaldehyde-crosslinked, lysed, sonicated, precipitated, and reverse crosslinked. Illumina sample libraries were prepared according to NEB protocol E6040

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
19236382
Reads aligned (%)
22.4
Duplicates removed (%)
3.1
Number of peaks
7150 (qval < 1E-05)

hg38

Number of total reads
19236382
Reads aligned (%)
22.8
Duplicates removed (%)
2.0
Number of peaks
7050 (qval < 1E-05)

Base call quality data from DBCLS SRA