Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
Glioblastoma
chip antibody
Brd4
antibody catalog number
Bethyl A301-985A
cell type
U87
duration
6h
concentration
500nM
drug
JQ1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
58712187
Reads aligned (%)
50.2
Duplicates removed (%)
47.7
Number of peaks
60712 (qval < 1E-05)

hg38

Number of total reads
58712187
Reads aligned (%)
52.6
Duplicates removed (%)
45.5
Number of peaks
61005 (qval < 1E-05)

Base call quality data from DBCLS SRA