Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

Embryoid Bodies

MeSH Description

Spontaneous aggregations of human embryonic stem cells that occur in vitro after culturing in a medium that lacks LEUKEMIC INHIBITORY FACTOR. The embryoid bodies can further differentiate into cells that represent different lineages.

Sample

source_name

mcherry+ mesoderm

cell type

ES cell-derived embryoid bodies

strain

C57Bl/6 X Sv136

ebs stage

day 4

genotype

Scl wt

antibody

none

chiprx

spike in drosophila S2 cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Complexes were eluted from beads in a thermomixer at 65 degres for 15 min and the eluates and reserved inputs were reverse cross-linked overnight at 65C. The samples were then treated with RNaseA, proteinase K and phenol/chloroform purified. ChIP samples were submitted to library preparation using NEBNext Ultra DNA Library prep kit for Illumina (NEB 7370) and multiplexing barcodes (NEB E7500) following Illumina's instructions.

Sequencing Platform

instrument_model

NextSeq 550

mm10

Number of total reads

39627622

Reads aligned (%)

81.8

Duplicates removed (%)

6.8

Number of peaks

399 (qval < 1E-05)

mm9

Number of total reads

39627622

Reads aligned (%)

81.7

Duplicates removed (%)

7.6

Number of peaks

396 (qval < 1E-05)