Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pancreas

Cell type

Pancreatic acinar cells

NA

NA

Sample

source_name

Pancreatic acinar cells

strain

C57BL6

genotype

Mist1-/-

antibody

Input

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Isolated nuclei from acinar cells were lysed and sonicated to yield sheared chromatin. Histone-DNA complexes were isolated with H3K4Me3 antibody antibody. Libraries were prepared according to established standard protocol at BGI (HK) .

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

12000000

Reads aligned (%)

99.1

Duplicates removed (%)

13.7

Number of peaks

304 (qval < 1E-05)

mm9

Number of total reads

12000000

Reads aligned (%)

98.8

Duplicates removed (%)

13.9

Number of peaks

328 (qval < 1E-05)