Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
cic

Cell type

Cell type Class
Embryo
Cell type
2-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
2-4h gd7 embryo
genotype/variation
gd7
tissue
embryo
chip antibody
anti-cic rabbit polyclonal, custom (GenScript)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed following He et al. (2011) ChIP-seq and ChIP-nexus was performed according to He et al. (2011) and He et al. (2015) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
24159113
Reads aligned (%)
54.5
Duplicates removed (%)
40.5
Number of peaks
2511 (qval < 1E-05)

dm6

Number of total reads
24159113
Reads aligned (%)
50.1
Duplicates removed (%)
45.0
Number of peaks
2624 (qval < 1E-05)

Base call quality data from DBCLS SRA