Antigen

Antigen Class

TFs and others

Antigen

cic

Cell type

Cell type Class

Embryo

Cell type

2-4h embryos

NA

NA

Sample

source_name

2-4h gd7 embryo

genotype/variation

gd7

tissue

embryo

chip antibody

anti-cic rabbit polyclonal, custom (GenScript)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed following He et al. (2011) ChIP-seq and ChIP-nexus was performed according to He et al. (2011) and He et al. (2015) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm3

Number of total reads

24159113

Reads aligned (%)

91.5

Duplicates removed (%)

40.5

Number of peaks

2511 (qval < 1E-05)

dm6

Number of total reads

24159113

Reads aligned (%)

91.1

Duplicates removed (%)

45.0

Number of peaks

2624 (qval < 1E-05)