Antigen

Antigen Class

TFs and others

Antigen

dl

Cell type

Cell type Class

Embryo

Cell type

2-4h embryos

NA

NA

Sample

source_name

2-4h toll10b embryo

genotype/variation

toll10b

tissue

embryo

chip antibody

anti-dl rabbit polyclonal, custom (GenScript)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed following He et al. (2011) ChIP-seq and ChIP-nexus was performed according to He et al. (2011) and He et al. (2015) with whole cell extract (WCE) derived from 100 mg embryos per ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm3

Number of total reads

18955656

Reads aligned (%)

93.2

Duplicates removed (%)

12.6

Number of peaks

2666 (qval < 1E-05)

dm6

Number of total reads

18955656

Reads aligned (%)

92.4

Duplicates removed (%)

16.8

Number of peaks

3515 (qval < 1E-05)