Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cardiovascular

Cell type

Endothelium, Lymphatic

MeSH Description

Unbroken cellular lining (intima) of the lymph vessels (e.g., the high endothelial lymphatic venules). It is more permeable than vascular endothelium, lacking selective absorption and functioning mainly to remove plasma proteins that have filtered through the capillaries into the tissue spaces.

Sample

source_name

Lymphatic endothelial cell

strain

C57BL/6

tissue

Skin

cell type

Mouse primary dermal lymphatic endothelial cell

passages

4-5

treatment

DMSO

chip antibody

input

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The primary mouse dermal LECs derived from C57BL/6 were were maintained in CellBiologics culture medium supplemented with VEGF, ECGS, Heparin, EGF, Hydrocortisone, L-glutamine, Antbiotic-Antimycotic solution, FBS). Approximately 1-3 x 10^7 cells were used for each ChIP-Seq assay. The chromatin was precipitated by H3K79me2 antibody (ab3594, Abcam) and immunoprecipitated DNAs were purified with treatment of proteinase K, phenol/chloroform and ethanol precipitation. ChIP-Seq libraries were produced using Truseq ChIP Sample kit (Illumina) according to manufacturer's instruction.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

47652227

Reads aligned (%)

95.3

Duplicates removed (%)

40.4

Number of peaks

493 (qval < 1E-05)

mm9

Number of total reads

47652227

Reads aligned (%)

94.9

Duplicates removed (%)

40.3

Number of peaks

596 (qval < 1E-05)