GSM1192072: IgG.shRECQL5-5; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293 cells, IgG.shRECQL5-5
cell line
HEK293
chip antibody
Non-specific IgG control
treatment
RECQL5 shRNA 5
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with formaldehyde 1% final. Nuclei were extracted in low salt conditions and burst in high salt before shearing the DNA to reduce the size to 300-400 bp and performing ChIP with an RNA Pol II specific antibody. DNA libraries were prepared according to Illumina's instructions and run on a GAIIX sequencer