Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Blood

Cell type

OCI-AML3

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

Brd4-CHIP-JQ1

cell line

NPMc+ leukemia OCI-AML3 cells

cell type

acute myeloid leukemia

treatment

JQ1, 500 nM for 24 h

chip antibody

Brd4 (A301-985A50, Lot #, 6, BETHYL Laboratories)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) was performed as described previously (Lee TI, Johnstone SE, Young RA. Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc. 2006;1(2):729-748) ChIP-seq libraries were prepared according to the Illumina protocol for ChIP-seq

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

21092512

Reads aligned (%)

86.5

Duplicates removed (%)

26.9

Number of peaks

991 (qval < 1E-05)

hg19

Number of total reads

21092512

Reads aligned (%)

85.4

Duplicates removed (%)

29.9

Number of peaks

840 (qval < 1E-05)