Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
OCI-AML3
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Brd4-CHIP-JQ1
cell line
NPMc+ leukemia OCI-AML3 cells
cell type
acute myeloid leukemia
treatment
JQ1, 500 nM for 24 h
chip antibody
Brd4 (A301-985A50, Lot #, 6, BETHYL Laboratories)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as described previously (Lee TI, Johnstone SE, Young RA. Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc. 2006;1(2):729-748) ChIP-seq libraries were prepared according to the Illumina protocol for ChIP-seq

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21092512
Reads aligned (%)
59.8
Duplicates removed (%)
29.9
Number of peaks
840 (qval < 1E-05)

hg38

Number of total reads
21092512
Reads aligned (%)
63.2
Duplicates removed (%)
26.9
Number of peaks
991 (qval < 1E-05)

Base call quality data from DBCLS SRA