Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Astrocytes

MeSH Description

A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Sample

source_name

astrocyte

cell type

astrocyte

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted using phenol chlorophorm. Cells were accutased, washed and immediately fixed with 1% formaldehyde. Chromatin was sonicated and immunoprecipitated with Rad21 antibody and PGS beads. Immunoprecipitated material was ligated to PE adapters. Libraries were amplified, checked on Bioanalyser and sequenced.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

79798223

Reads aligned (%)

95.0

Duplicates removed (%)

19.6

Number of peaks

964 (qval < 1E-05)

mm9

Number of total reads

79798223

Reads aligned (%)

94.6

Duplicates removed (%)

19.4

Number of peaks

1108 (qval < 1E-05)