Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Neural Stem Cells
MeSH Description
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Attributes by original data submitter

Sample

source_name
neural stem cell
cell type
neural stem cell
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using phenol chlorophorm. Cells were accutased, washed and immediately fixed with 1% formaldehyde. Chromatin was sonicated and immunoprecipitated with Rad21 antibody and PGS beads. Immunoprecipitated material was ligated to PE adapters. Libraries were amplified, checked on Bioanalyser and sequenced.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
88437654
Reads aligned (%)
94.6
Duplicates removed (%)
21.1
Number of peaks
23105 (qval < 1E-05)

mm9

Number of total reads
88437654
Reads aligned (%)
94.2
Duplicates removed (%)
20.9
Number of peaks
22311 (qval < 1E-05)

Base call quality data from DBCLS SRA