Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MED1

Cell type

Cell type Class
Others
Cell type
Mesenchymal stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
hMSC-TERT4_MED1_D1
cell line
hMSC-TERT4
differentiation stage
D1
chip antibody
MED1/TRAP220 antibody (M-255) Cat# SC-8998

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed according to standard protocol as described in (Siersbæk et al. 2012, MCB, 32: 3452-3463) Libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014, Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

hg19

Number of total reads
24874705
Reads aligned (%)
68.8
Duplicates removed (%)
29.3
Number of peaks
2505 (qval < 1E-05)

hg38

Number of total reads
24874705
Reads aligned (%)
71.5
Duplicates removed (%)
27.2
Number of peaks
2537 (qval < 1E-05)

Base call quality data from DBCLS SRA