Antigen

Antigen Class

TFs and others

Antigen

MED1

Cell type

Cell type Class

Others

Cell type

Mesenchymal stem cells

NA

NA

Sample

source_name

hMSC-TERT4_MED1_D0

cell line

hMSC-TERT4

differentiation stage

D0

chip antibody

MED1/TRAP220 antibody (M-255) Cat# SC-8998

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed according to standard protocol as described in (Siersbæk et al. 2012, MCB, 32: 3452-3463) Libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014, Methods in Enzymology 2014, Vol. 537, pp. 261-279).

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

26123648

Reads aligned (%)

98.0

Duplicates removed (%)

33.7

Number of peaks

4210 (qval < 1E-05)

hg19

Number of total reads

26123648

Reads aligned (%)

97.1

Duplicates removed (%)

35.3

Number of peaks

4195 (qval < 1E-05)