Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 breast cancer cell line
cell type
MCF-7 breast cancer cell line
chip antibody
ERα (sc543; Santa Cruz Biotechnology)
chip treatment conditions
10nM E2, 45 minutes

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP of DNA was performed as described in Schmidt et al., Methods (2009) doi: 10.1016/j.ymeth.2009.03.001 Illumina sequencing libraries were prepared as described in Schmidt et al., Methods (2009) doi: 10.1016/j.ymeth.2009.03.001

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
33768557
Reads aligned (%)
44.9
Duplicates removed (%)
52.5
Number of peaks
16657 (qval < 1E-05)

hg38

Number of total reads
33768557
Reads aligned (%)
46.4
Duplicates removed (%)
51.3
Number of peaks
16808 (qval < 1E-05)

Base call quality data from DBCLS SRA