Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2 cells

cell line

HepG2

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cross-linked chromatin was immunoprecipitated with an antibody. The Protein:DNA crosslinks were then reversed and the DNA fragments were recovered and sequenced. Please see protocol notes below and go to http://hudsonalpha.org/myers-lab/protocols for the most current version of the protocol. Illumina Single End for ChIP-seq

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

77022808

Reads aligned (%)

97.6

Duplicates removed (%)

4.9

Number of peaks

1944 (qval < 1E-05)

hg19

Number of total reads

77022808

Reads aligned (%)

96.9

Duplicates removed (%)

6.1

Number of peaks

1737 (qval < 1E-05)