Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tbx4

Cell type

Cell type Class
Embryo
Cell type
Hindlimb
MeSH Description
Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)

Attributes by original data submitter

Sample

source_name
e11.5 HL
Stage
e11.5
tissue
hindlimb
genotype
wild type
strain
CD1
chip antibody
homemade anti-Tbx4 (1695a)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was fixed with 1% formaldehyde, nuclei were isolated, genomic DNA was sheared by sonication, and protein:DNA complexes were pulled down with the indicated antibodies. Illumina

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
77853090
Reads aligned (%)
84.0
Duplicates removed (%)
84.6
Number of peaks
703 (qval < 1E-05)

mm9

Number of total reads
77853090
Reads aligned (%)
83.9
Duplicates removed (%)
84.5
Number of peaks
645 (qval < 1E-05)

Base call quality data from DBCLS SRA