Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
immortalized mouse embryonic fibroblast cell line
cell line
R26M2rtTA/+;TetOcre;Sdhcfl/fl
day
16
antibody
none (input control)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells harvested by trypsinization and fixed with formaldehyde prior to lysis. Chromatin prepared via MNase digestion and sonication. Either ChIP-enriched chromatin or non-enriched input chromatin used for library construction. ThruPLEX DNA-seq 48D Kit (Rubicon Genomics cat# R400406)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
32272655
Reads aligned (%)
98.2
Duplicates removed (%)
12.9
Number of peaks
172 (qval < 1E-05)

mm9

Number of total reads
32272655
Reads aligned (%)
98.0
Duplicates removed (%)
13.3
Number of peaks
146 (qval < 1E-05)

Base call quality data from DBCLS SRA