Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

Monocyte from peripheral blood of HIDS patients

cell type

Monocyte

treatment protocol

none

disease

Hyper IgD Syndrome (HIDS)

tissue

Adult Peripheral Blood

donor

HIDS_patient_3

chip antibody

Rabbit polyclonal anti-H3K27ac (Diagenode, cat. # pAb-196-050)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted using the RNEasy mini kit. ChIP was performed using BLUEPRINT protocols. Libraries were prepared using the KAPA hyperprep kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

28749007

Reads aligned (%)

96.1

Duplicates removed (%)

21.8

Number of peaks

13055 (qval < 1E-05)

hg19

Number of total reads

28749007

Reads aligned (%)

95.4

Duplicates removed (%)

22.2

Number of peaks

12997 (qval < 1E-05)