Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

Control monocyte sample from peripheral blood of healthy volunteer

cell type

Monocyte

treatment protocol

none

disease

healthy

tissue

Adult Peripheral Blood

donor

Control_3

chip antibody

Rabbit polyclonal anti-H3K27ac (Diagenode, cat. # pAb-196-050)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted using the RNEasy mini kit. ChIP was performed using BLUEPRINT protocols. Libraries were prepared using the KAPA hyperprep kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

29029327

Reads aligned (%)

96.2

Duplicates removed (%)

23.8

Number of peaks

14891 (qval < 1E-05)

hg19

Number of total reads

29029327

Reads aligned (%)

95.6

Duplicates removed (%)

24.1

Number of peaks

14822 (qval < 1E-05)