Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NOTCH3

Cell type

Cell type Class
Blood
Cell type
T-ALL
NA
NA

Attributes by original data submitter

Sample

source_name
T-ALL1_DMSO
cell type
T-Acute Lymphoblastic Leukemia patient derived cell line (T-ALL1)
treated with
vehicle (DMSO) for 1 day
chip antibody
Notch3 ChIP-seq
chip antibody
anti-NOTCH3 antibody (Cell Signaling, #2889)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using EZ-Magna ChIP (Millipore) according to the manufacturers protocol. In brief, cells were fixed, sheared by sonication and immunoprecipitated with anti-NOTCH3 antibody (Cell Signaling, #2889), or anti-H3K27Ac antibody (Abcam, ab4729). Libraries were constructed using an NEB NEXT kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21717527
Reads aligned (%)
86.7
Duplicates removed (%)
10.8
Number of peaks
1090 (qval < 1E-05)

hg38

Number of total reads
21717527
Reads aligned (%)
88.4
Duplicates removed (%)
9.7
Number of peaks
899 (qval < 1E-05)

Base call quality data from DBCLS SRA