Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
T-ALL
NA
NA

Attributes by original data submitter

Sample

source_name
T-ALL1_DMSO
cell type
T-Acute Lymphoblastic Leukemia patient derived cell line (T-ALL1)
treated with
vehicle (DMSO) for 1 day
chip antibody
H3K27Ac ChIP-seq
chip antibody
anti-H3K27Ac antibody (Abcam, ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using EZ-Magna ChIP (Millipore) according to the manufacturers protocol. In brief, cells were fixed, sheared by sonication and immunoprecipitated with anti-NOTCH3 antibody (Cell Signaling, #2889), or anti-H3K27Ac antibody (Abcam, ab4729). Libraries were constructed using an NEB NEXT kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
87770293
Reads aligned (%)
97.9
Duplicates removed (%)
26.9
Number of peaks
86456 (qval < 1E-05)

hg19

Number of total reads
87770293
Reads aligned (%)
97.3
Duplicates removed (%)
27.9
Number of peaks
86024 (qval < 1E-05)

Base call quality data from DBCLS SRA