GSM2793825: T-ALL1 DMSO treated H3K27Ac ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
T-ALL
NA
NA
Attributes by original data submitter
Sample
source_name
T-ALL1_DMSO
cell type
T-Acute Lymphoblastic Leukemia patient derived cell line (T-ALL1)
treated with
vehicle (DMSO) for 1 day
chip antibody
H3K27Ac ChIP-seq
chip antibody
anti-H3K27Ac antibody (Abcam, ab4729)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using EZ-Magna ChIP (Millipore) according to the manufacturers protocol. In brief, cells were fixed, sheared by sonication and immunoprecipitated with anti-NOTCH3 antibody (Cell Signaling, #2889), or anti-H3K27Ac antibody (Abcam, ab4729). Libraries were constructed using an NEB NEXT kit.