Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
E6.5 embryos
NA
NA

Attributes by original data submitter

Sample

source_name
K27me3 microChIP
strain
ICR
developmental stage
6.5 dpc
tissue
embryo
chip antibody
H3K27me3 (merck millipore, cat. 07-449, lot 2275589)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA acquired from ChIP assay (H3K27me3, merck millipore, catlog number: 07-449, lot number: 2275589) of embryo samples were separated and extracted by microChIP. Libraries of genomic DNA were construted by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB #7370L).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
22478994
Reads aligned (%)
98.4
Duplicates removed (%)
51.7
Number of peaks
428 (qval < 1E-05)

mm9

Number of total reads
22478994
Reads aligned (%)
98.3
Duplicates removed (%)
51.6
Number of peaks
394 (qval < 1E-05)

Base call quality data from DBCLS SRA