Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
E6.5 embryos
NA
NA

Attributes by original data submitter

Sample

source_name
genomic input sequencing
strain
ICR
developmental stage
6.5 dpc
tissue
embryo

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Genomic DNA acquired from ChIP assay (H3K27me3, merck millipore, catlog number: 07-449, lot number: 2275589) of embryo samples were separated and extracted by microChIP. Libraries of genomic DNA were construted by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB #7370L).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
29797342
Reads aligned (%)
98.4
Duplicates removed (%)
12.0
Number of peaks
253 (qval < 1E-05)

mm9

Number of total reads
29797342
Reads aligned (%)
98.3
Duplicates removed (%)
11.9
Number of peaks
240 (qval < 1E-05)

Base call quality data from DBCLS SRA