Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Monocytes
MeSH Description
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Attributes by original data submitter

Sample

source_name
Adult Human Peripheral Blood
disease status
Healthy
cell type
Monocyte
treatment
Bacillus Calmette-Guérin (BCG) vaccine
time point
1 month
donor
15
chip antibody
Rabbit polyclonal anti-H3K27ac (Diagenode, cat. # pAb-196-050)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was extracted using the RNeasy mini kit. ChIP was performed using BLUEPRINT protocols. Libraries were prepared using the KAPA hyperprep kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
29815960
Reads aligned (%)
96.2
Duplicates removed (%)
4.9
Number of peaks
14861 (qval < 1E-05)

hg19

Number of total reads
29815960
Reads aligned (%)
95.6
Duplicates removed (%)
5.1
Number of peaks
14724 (qval < 1E-05)

Base call quality data from DBCLS SRA