GSM2790511: (HN)-SCC25 SOX2 ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SOX2
Cell type
Cell type Class
Others
Cell type
Head and neck squamous cell carcinoma
NA
NA
Attributes by original data submitter
Sample
source_name
Head and neck squamous cell carcinoma
tissue
tongue
antibody
anti-Sox2 (R&D, AF2018), 4ug.
condition
Cells were grown DMEM-F12 media 10% FBS
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared from 2x10^7 SCC cells or 1x10^6 sorted cells using the ChIP-IT High Sensitivity kit (53040, Active Motif) according to manufacturers instructions. Library prep was performed with ThruPLEX DNA-seq kit (Rubicon Genomics, R400427) using 200pg of ChIP DNA or KAPA Hyper Prep kit (KAPA, KK8505) using 1ng of ChIP DNA