Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Others

Cell type

Head and neck squamous cell carcinoma

NA

NA

Sample

source_name

Head and neck squamous cell carcinoma

tissue

tongue

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was prepared from 2x10^7 SCC cells or 1x10^6 sorted cells using the ChIP-IT High Sensitivity kit (53040, Active Motif) according to manufacturers instructions. Library prep was performed with ThruPLEX DNA-seq kit (Rubicon Genomics, R400427) using 200pg of ChIP DNA or KAPA Hyper Prep kit (KAPA, KK8505) using 1ng of ChIP DNA

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

43915469

Reads aligned (%)

4.0

Duplicates removed (%)

41.6

Number of peaks

28740 (qval < 1E-05)

hg19

Number of total reads

43915469

Reads aligned (%)

3.8

Duplicates removed (%)

39.8

Number of peaks

31034 (qval < 1E-05)