Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MORC2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
ChIP:MORC2_Cell_hESC:WT
cell line
hESC
cell type
Human embryonic stem cell lines
genotype/variation
wild type
chip antibody
MORC2 (A300-149A, Bethyl Laboratories)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Total RNA was isolated from K562 cells using the RNAeasy kit (Qiagen # 74104) and treated with RNase-Free DNase Set (Qiagen # 79254) to remove genomic DNA, according to the manufacturer’s instructions. ChIP DNA, input DNA or cDNA were subject to end repair, A-tailing, adaptor ligation and cleavage with USER enzyme, followed by size selection to 250-500 bp and amplification with NEBNext sequencing primers. Libraries were purified, quantified, multiplexed and sequenced with 1x75bp single-end reads on an Illumina NEXT-seq (Stanford Functional Genomics Facility).

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
84103205
Reads aligned (%)
54.4
Duplicates removed (%)
72.0
Number of peaks
1861 (qval < 1E-05)

hg38

Number of total reads
84103205
Reads aligned (%)
56.2
Duplicates removed (%)
71.3
Number of peaks
2755 (qval < 1E-05)

Base call quality data from DBCLS SRA