GSM2789812: ChIP:MPP8 Cell hESC:WT rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
MPHOSPH8
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ChIP:MPP8_Cell_hESC:WT
cell line
hESC
cell type
Human embryonic stem cell lines
genotype/variation
wild type
chip antibody
MPP8 (16796-1-AP, Protein Technologies Inc)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Total RNA was isolated from K562 cells using the RNAeasy kit (Qiagen # 74104) and treated with RNase-Free DNase Set (Qiagen # 79254) to remove genomic DNA, according to the manufacturer’s instructions. ChIP DNA, input DNA or cDNA were subject to end repair, A-tailing, adaptor ligation and cleavage with USER enzyme, followed by size selection to 250-500 bp and amplification with NEBNext sequencing primers. Libraries were purified, quantified, multiplexed and sequenced with 1x75bp single-end reads on an Illumina NEXT-seq (Stanford Functional Genomics Facility).