GSM1184312: Input DP (for Input DP for Nipbl and Med1 ChIP); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
CD4 CD8 double positive cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD4+ CD8+ Double Positive Thymocytes
cell type
CD4+ CD8+ Double Positive Thymocytes
chip antibody
input DNA
genotype
wild type
genetic background
C57BL\6 and 129 mixed
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sorted CD4+ CD8+ DP thymocytes were fixed in 10% FCS DMEM media containing 1% formaldehyde. The cells were lysed and sonicated in [20 mM Tris-HCl, pH8, 150 mM NaCl, 2mM EDTA, 0.1% SDS, 1% Triton X, protease inhibitors]. Lysates were cleared by centrifugation and incubated with a mixture of RNaseA and proteinaseK for 2 hours at 37° and then 16 hours at 65° to reverse the cross-links. DNA was purified by phenol-chloroform extraction and 10ng was used with a Illumina ChIP-seq sample preparation according to the manufacturer instructions.