Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
mouse macrophage
cell type
bone marrow-derived immortalized macrophage
treatment
none
strain
C57BL/6
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and LXR-DNA or H3K27ac-DNA complexes were isolated with indicated antibodies. Libraries were prepared using the NEBNext Ultra DNA Library Prep kit for Illumina (Ref.:#7370) following the manufacture instructions. 12 cycles of PCR were done for the final library amplification for all samples.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
44154111
Reads aligned (%)
96.5
Duplicates removed (%)
13.5
Number of peaks
402 (qval < 1E-05)

mm9

Number of total reads
44154111
Reads aligned (%)
96.2
Duplicates removed (%)
13.5
Number of peaks
456 (qval < 1E-05)

Base call quality data from DBCLS SRA