Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
Mammary glands
NA
NA

Attributes by original data submitter

Sample

source_name
Mammary gland tissues, p6, input
strain/background
129SV::FVB/N::C57BL/6
genotype/variation
wild type
tissue
mammary gland
developmental stage
day 6 of pregnancy
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ChIP DNA fragments were blunt-ended and ligated to the Illumina Indexed DNA adaptors using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (# E6240, New England BioLabs, Ipswich, MA), and sequenced using the Illumina HiSeq 2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
47915663
Reads aligned (%)
98.3
Duplicates removed (%)
88.7
Number of peaks
208 (qval < 1E-05)

mm9

Number of total reads
47915663
Reads aligned (%)
98.1
Duplicates removed (%)
88.8
Number of peaks
202 (qval < 1E-05)

Base call quality data from DBCLS SRA