Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Meis1

Cell type

Cell type Class
Blood
Cell type
Myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
Hoxa9/Meis1 AML
cell type
Hoxa9-and Meis1-co-expressing murine AML cells
strain
C57BL/6
chip antibody
Meis1 (Abcam, ab19867, lot 676286)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and immunoprecipitated with each antibodies Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

mm10

Number of total reads
7149796
Reads aligned (%)
39.2
Duplicates removed (%)
8.7
Number of peaks
4638 (qval < 1E-05)

mm9

Number of total reads
7149796
Reads aligned (%)
39.1
Duplicates removed (%)
9.2
Number of peaks
4621 (qval < 1E-05)

Base call quality data from DBCLS SRA