Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Bone
Cell type
VA-ES-BJ
Primary Tissue
Bone
Tissue Diagnosis
Sarcoma

Attributes by original data submitter

Sample

source_name
VA-ES-BJ (EpS)
antibody
H3K4me3
antibody vendor/catalog
Millipore 05-745R

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed per standard protocols (Millipore, Billerica, MA) with minor modifications. Briefly, 10M cells were crosslinked with 1% formaldehyde for 10 min at 37 °C. This reaction was subsequently quenched with 0.125M glycine. All library prep and sequencing were 75bp single end on Illuminia Nextseq 500

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
8534247
Reads aligned (%)
95.8
Duplicates removed (%)
4.5
Number of peaks
19396 (qval < 1E-05)

hg19

Number of total reads
8534247
Reads aligned (%)
95.4
Duplicates removed (%)
4.9
Number of peaks
19389 (qval < 1E-05)

Base call quality data from DBCLS SRA