GSM2781968: Non mig Input Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Uterus
Cell type
SiHa
Primary Tissue
Cervix
Tissue Diagnosis
Carcinoma Squamous Cell
Attributes by original data submitter
Sample
source_name
SiHa cell line
tissue type
Squamous cell carcinoma of the cervix (primary site)
passages
18-23
cell line
SiHa
antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated chromatin was centrifuged to clarify debris, and DNA-antibody complexes were immunoprecipitated using antibody to H3K9me3 (abcam 8898, lot number GR232099-3) Libraries for replicate1 were constructed using Illumina TruSeq ChIP Sample Prep kit (cat no 15027084) with gel extraction. As a high degree of duplication was noted in this replicate, likely due to DNA losses during gel extraction, library preparation for a second biological replicate (replicate2), was performed with the help of Ampure XP beads, using NEB Ultra DNA library kit (Ref E7370L). This resulted in much lower duplication rates. Analysis was performed on replicate2, and major findings were confirmed in replicate1 after deduplication.