Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
strain
129/Ola
chip antibody
H3K27me2 (Cell Signaling, Rabbit mAb, D18C8)
genotype
WT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
NEBNext Ultra II protocol (NEB, E7645S)

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
35330982
Reads aligned (%)
96.1
Duplicates removed (%)
7.7
Number of peaks
365 (qval < 1E-05)

mm9

Number of total reads
35330982
Reads aligned (%)
96.0
Duplicates removed (%)
7.8
Number of peaks
384 (qval < 1E-05)

Base call quality data from DBCLS SRA